Melanops tulasnei Fuckel, Jb. nassau. Ver. Naturk. 23-24: 225 (1870) [1869-70]
Saprobic on dead twigs of Quercus robur. Sexual morph: see Phillips and Alves (2009) and Phillips et al. (2013). Asexual morph: Conidiomata 1000–1500 µm diam., 500–750 µm high, dark brown to black, stromatic, mostly solitary, occasionally gregarious, subepidermal, semi-immersed to partly erumpent, rounded in surface view, multi-locular, thick-walled, glabrous, ostiolate, with locules 100–400 µm diam., 200–550 µm high, globose to subglobose, divided by pale brown to hyaline wall. Ostiole 80–150 × 50–100 µm, multi-ostiolar, circular, papillate. Conidiomatal wall 20–105 µm wide, composed an outer layer of textura globosa to textura angularis with relatively thick-walled, dark brown to black cells, and an inner layer of textura prismatica with thick-walled, pale brown to hyaline cells. Paraphyses 100–150 × 1–2 µm, hyaline, conspicuous, filiform, septate, branched irregularly, arising between conidiophores. Conidiophores reduced to conidiogenous cells, lining the base and side walls of conidiomata. Conidiogenous cells 20–40 × 1.5–3.5 µm, hyaline, holoblastic, cylindrical to subcylindrical, determinate, smooth-walled. Conidia 53–85 × 8–11 µm ( = 61 × 9 µm; n = 30), hyaline or occasionally pale brown, elongate fusiform, attenuating towards each end, obtuse at the apex and truncate at the base, unicellular, enclosed in mucus sheath.
Culture characteristics: Colonies reaching 15–20 mm diam. after four weeks at 20–25°C, white at first, then becoming grey or olivaceous, eventually black, circular to irregular, raised, with aerial hyphae, dense, filamentous, cottony; margins undulate to lobate, reverse white at first, then becoming black; sporulation after 12 months.
Material examined: Russia, on dead twigs of Quercus robur (Fagaceae), 8 May 2014, T.S. Bulgakov, T48 (MFLU 19-2889, living culture MFLUCC 16-1194, (KUN, HKAS 101667).
Notes: Strain (MFLUCC 16-1194) clustered with two strains of M. tulasnei (CBS 116806, CBS 116805). Immature asci were found in the same stroma with the asexual morph. The asexual morph, except paraphyses and conidial dimensions which are much larger than the epitype strain M. tulasnei (37.2–)45–46.8(–53) × (7.2–)9.1–9.7 (–12.1), is in other aspects almost similar. Phillips and Alves (2009) noted that the conidial dimensions of M. tulasnei is variable and the difference can be regarded as intra-species variation. In addition, the range of conidial dimension of M. fagicola and M. tulasnei overlap, but they are phylogenetically distinct. Therefore, the new collection is recognized as conspecific with M. tulasnei, and the difference might be caused by different geography. Here, we note that conidial dimensions alone are not informative at the species level in Melanops.
Li WJ, McKenZie EHC, Liu JK, Bhat DJ, Dai DQ, Caporesi E, Tian Q, Maharachcikumbura SSN, Luo ZL, Shang QJ, Zhang JF, Tangthirasunun N, Karunarathna SC, Xu JC, Hyde KD (2020) Taxonomy and phylogeny of hyaline-spored coelomycetes. Fungal Diversity 100: pages279–801.