Darkera picea H.S. Whitney, J. Reid & Piroz., Can. J. Bot. 53(24): 3053 (1975)
Saprobic on dead needles of Picea excels, dark brown fruiting bodies, in a linear series. Sexual morph: undetermined. Asexual morph: Conidiomata 250–400 µm high, 200–300 µm diam., pycnidial, separate or gregarious, globose, subglobose to ovoid, immersed, subepidermal, unilocular, glabrous, ostiolate, thick-walled. Conidiomatal wall 20–35 µm wide, composed of cells of outer textura intricata and inner textura epidermoidea, thick-walled, dark brown to pale brown. Ostiole 20–40 µm wide, centrally or laterally located, long, subcylindrical, single or sometimes 2-ostiolate. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 3–6.5 µm long × 3–7 µm wide, hyaline, holoblastic, lageniform to subcylindrical, smooth, arising from inner layers of conidiomata. Conidia 20–28 × 3.5–4.5 µm (= 25.5 × 4 µm; n = 30), subcylindrical, straight or slightly curved, with a rounded apex, and narrow and truncate base often with minute frills, unicellular, hyaline, smooth, thick-walled, bearing an irregular, widely flared, undulated, mucoid, apical appendage resulting from the eversion of a mucoid sheath enveloping the upper part of developing conidium.
Material examined: Italy, Province of Trento, Mezzana, Marilleva 1400, on dead and not land needles of Picea abies (Pinaceae), 20 August 2014, Erio Camporesi, IT2050 (KUN, HKAS 93593), IT2050A (MFLU 19-2868).
Notes: Our collection is morphologically conforms to the type of Darkera picea.
Darkera picea (HKAS 93593) a Material specimen. b Black coniodiomata on the host. c–e Vertical section of conidioma. f Section of peridium. g Ostiole. h–j Conidiogenous cells and developing conidia. k–o Conidia. Scale bars: a = 1000 µm, b = 200 µm, c = 100 µm, d–e, g = 50 µm, f = 20 µm, h–j = 10 um, k–o = 5 µm.
Li WJ, McKenZie EHC, Liu JK, Bhat DJ, Dai DQ, Caporesi E, Tian Q, Maharachcikumbura SSN, Luo ZL, Shang QJ, Zhang JF, Tangthirasunun N, Karunarathna SC, Xu JC, Hyde KD (2020) Taxonomy and phylogeny of hyaline-spored coelomycetes. Fungal Diversity 100: pages279–801.