Darkera pseudotsugae (H.S. Whitney, J. Reid & Piroz.) Crous, in Crous et al., Phytotaxa 202(2): 85 (2015)
≡ Tiarosporella pseudotsugae H.S. Whitney, J. Reid & Piroz., Can. J. Bot. 53(24): 3057 (1975)
Facesoffungi number: FoF 07268
Foliicolous, occurring on leaves of Pseudotsuga menziesii. Sexual morph: undetermined. Asexual morph: Conidiomata 200–250 µm diam., 150–200 µm high, brown to dark brown, hypophyllous, pycnidial, solitary to gregarious, subepidermal, deeply immersed, globose to subglobose, unilocular, glabrous, thick-walled, ostiolate. Ostiole single, cylindrical to circular, centrally located. Conidiomatal wall 30–50 µm wide, composed of thick-walled, pale brown cells of textura intricata in the basal part, passing into relatively thick-walled, brown to hyaline cell of textura intracata mixed with textura epidermoidea in the upper half part, becoming dark brown textura intracata at ostiolar region. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 5–15 × 3–6 µm, hyaline, enteroblastic, ampuliform to lageniform or conical, discrete or integrated, smooth-walled. Conidia 42–55 × 5–7.5 µm ( = 50 × 6 µm; n = 30), hyaline, cylindrical to clavate, with bulging and rounded apex, attenuated below to a narrow, truncate base, straight or slightly curved, unicellular, smooth-walled, bearing a funnel-shaped to irregular, mucoid appendage at apex, or occasionally a subcylindrical mucoid appendage at base.
Material examined: Canada, Alberta, Edith Cavell turnoff, Jasper, on leaf of Pseudotsuga menziesii (Pinaceae), 26 July 1974, R.J. Bourchier (DAOM 49190, holotype).
Darkera pseudotsugae (DAOM 49190, holotype) a, b Herbarium package and specimen. c, d Appearance of brown to dark brown conidiomata on the host. e Vertical section of conidiomata. f, h Section of peridium. g Ostiole. i–m Conidiogenous cells and developing conidia. n–q Conidia. Scale bars c = 500 µm, d = 200 µm, e = 100 µm, f, h, k = 20 µm, g = 50 µm, i–j = 10 µm, l, n–q = 10 µm, m = 5 µm.
Li WJ, McKenZie EHC, Liu JK, Bhat DJ, Dai DQ, Caporesi E, Tian Q, Maharachcikumbura SSN, Luo ZL, Shang QJ, Zhang JF, Tangthirasunun N, Karunarathna SC, Xu JC, Hyde KD (2020) Taxonomy and phylogeny of hyaline-spored coelomycetes. Fungal Diversity 100: pages279–801.