Phacidium anomala (Nag Raj) W.J. Li & K.D. Hyde.
≡ Ceuthospora anomala Nag Raj, Coelomycetous Anamorphs with Appendage-bearing Conidia (Ontario): 163 (1993)
Index Fungorum number: IF557172, Facesoffungi number: FoF 07501
Saprobic on dead leaves of Osmanthus sp. (Oleaceae) and Dactylis (Poaceae). Sexual morph: undetermined. Asexual morph: Conidiomata 250–300 µm diam., 170–250 µm high, dark brown to black, stromatic, pycnidial, epiphyllous, amphigenous on leaves, solitary, gregarious or confluent, subepidermal, immersed to semi-immersed, globose to subglobose, multilocular, with 3–10 locules; individual locules subglobose, 250–300 µm diam., 170–250 µm high, each with an independent ostiole. Ostioles 25–35 µm wide, centrally located, circular, rounded. Conidiomata wall 30–45 µm wide, composed of two types of pseudoparenchymatous cells, forming several inner layers of hyaline, thick-walled cells of textura epidermoidea, and several outer layers of brown, thick-walled cells of textura angularis. Conidiophores 20–30 × 3–4 µm, arising from inner wall layer of the conidiomata, hyaline, subcylindrical, septate, sympodially branched at the base and above, thick-walled. Conidiogenous cells 5–7 × 3–5 µm, hyaline, cylindrical, integrated, determinate, smooth-walled. Conidia 20–30 × 3.5–4.5 µm (= 25 × 4 µm; n = 30), hyaline, cylindrical to subcylindrical, with obtuse apex, and narrow, truncate base, unicellular, thick- and smooth-walled, bearing a cone-shaped or irregular, undulated, mucoid apical appendage resulting from the eversion of a mucoid sheath enveloping the upper part of developing conidium.
Culture characters: Colonies on PDA, reaching 8–9 cm after 14 d at 25–30 °C, white to flavescent with age, ultimately becoming brown to dark brown, circular, flattened, dense, slightly cottony with aerial mycelium on the surface, rounded and smooth at margins, reverse pale brown. Mycelium composed of hyaline to pale brown, cylindrical, branched, septate hyphae. Sporulation appearing after 4 weeks. Conidiomata formed from mycelium, black, pycnidial, solitary or gregarious, globose or irregular, multilocular, thick-walled. Conidiophores and conidiogenous cells not observed. Conidia 12–14 × 2–4.6 µm (= 12 × 3 µm; n = 30), hyaline, cylindrical, unicellular, smooth-walled, guttulate, without mucoid apical appendage.
Material examined: China, Province of Yunnan, Kunming, on dead leaves of Osmanthus sp. (Oleaceae), 20 June 2015, Wen-Jing Li, WJL0080 (MFLU 19-2903, reference specimen designated here), living culture MFLUCC 16-1151 = ICMP 21556 = KUMCC 15-0650; ibid., on dead leaves of Dactylis sp. (Poaceae), 20 June 2015, Wen-Jing Li, living culture MFLUCC 16-1155 = ICMP 21555, WJL0080C1 (MFLU 19-2904).
Notes: Our collections are similar to P. anomala in having immersed to semi-immersed, multilocular conidiomata with independent ostioles, sympodially branched conidiophores and cylindrical to subcylindrical, unicellular conidia with a cone-shaped, mucoid apical appendage. The differences between our strains and P. anomala are conidiomatal and conidial dimensions.
Phacidium anomala (MFLU 19-2903, reference specimen) a Herbarium specimen. b, c Appearance of black coniodiomata on the host. d, e Sections of conidiomata. f, i Sections of peridium. g–h, j–k Conidiophores, conidiogenous cells and developing conidia. l–o Conidia. Scale bars: d = 100 µm, e = 200 µm, f–h = 20 µm, i = 10 µm, j–o = 5 um.