Neodidymelliopsis negundinis Manawasinghe, Camporesi & K.D. Hyde, in Hyde et al., Mycosphere 9(2): 295 (2018)
Facesoffungi number: FoF 03891
Saprobic on dead stems of Sophora japonica. Sexual morph: undetermined. Asexual morph: Conidiomata 310–350 µm diam., 130–190 µm high, black, pycnidial, solitary to gregarious or confluent, immersed in origin, becoming erumpent, unilocular or multi-locular, with locules 140–250 µm diam., 110–190 µm, globose to subglobose, thick-walled, glabrous, papillate. Ostiole short, single, circular to cylindrical, centrally located. Conidiomatal wall 15–40 µm wide, composed of thick-walled, brown, gradually merging with hyaline cells of textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 4–9 × 3–6 µm, arising from the inner cavity of conidiomata, hyaline, enteroblastic, phialidic, doliiform to ampulliform, determinate, smooth-walled. Conidia 4.8–7.5 × 3–4 µm ( = 6 × 3.7 µm; n = 30), hyaline, ellipsoidal to ovoid, obtuse at both ends, straight or slightly curved, aseptate, smooth-walled, guttulate.
Culture characters: colonies on PDA, reaching 40 mm diam. after 4 weeks at 25–30°C, with brown to olivaceous, flattened, dense, filamentous, aerial, fluffy hyphae on the surface; margins white, circular, entire; reverse olivaceous.
Material examined: Italy, Province of Forlì-Cesena, Forlì, Via Pietro Nenni, on dead aerial branches of Sophora japonica (Fabaceae), 3 April 2016, Erio Camporesi, IT2907 (MFLU 16-1123), living culture MFLUCC 16-1316 = KUMCC 16-0091.
Notes: Neodidymelliopsis negundinis was original described from dead and dying twigs and branches of Acer negundo (Sapindaceae) in Russia (Hyde et al. 2018). Thus our collection is a new host record from Italy.
Neodidymelliopsis negundinis (MFLU 16-1123) a Herbarium specimen. b–d Appearance of brown conidiomata on the host. e–h Vertical sections of conidiomata. i, j Section of peridium. k–n Conidiogenous cells and developing conidia. o Germinating conidium. p–r Conidia. s Cultures on PDA. Scale bars b–c = 500 µm, d–e = 200 µm, f–h = 100 µm, i–j, o = 20 µm, k–n, p–r = 5 µm, s = 20 mm.
Li WJ, McKenZie EHC, Liu JK, Bhat DJ, Dai DQ, Caporesi E, Tian Q, Maharachcikumbura SSN, Luo ZL, Shang QJ, Zhang JF, Tangthirasunun N, Karunarathna SC, Xu JC, Hyde KD (2020) Taxonomy and phylogeny of hyaline-spored coelomycetes. Fungal Diversity 100: pages279–801.