Neopyrenochaeta annellidica W.J. Li, Z.H. Zhang & K.D. Hyde
Index Fungorum number: IF557155, Facesoffungi number: FoF 07481
Etymology: Referring to its annellidic conidiogenous cells.
Saprobic on submerged decaying wood in freshwater habitat. Sexual morph: undetermined. Asexual morph: Conidiomata 180–250 µm diam., 180–240 µm high, dark brown when moist, with white conidial mass surrounding ostiole, becoming black when dry, pycnidial, mostly solitary, occasionally gregarious, semi-immersed to superficial, globose to subglobose, unilocular, setose, ostiolate. Ostiole circular, papillate, centrally or laterally located. Setae up to 60 µm long, inconspicuous, pale brown to black, unbranched, septate, tapered toward apex, originating from the surface of the conidiomatal wall. Conidiomatal wall 10–40 µm wide, composed of thick-walled, brown to dark brown cells of textura angularis in the outer layers, becoming thinner, hyaline cells of textura prismatica towards conidial hymenium. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 7–20 × 5–9 µm, formed in the inner cavity of conidiomata, hyaline, enteroblastic, annellidic, doliiform to ampulliform, indeterminate, smooth-walled, with percurrent proliferation up to 2 times. Conidia 9–15 × 6–8 µm ( = 11 × 6.5 µm; n = 50), hyaline, oval or oblong, rounded at the apex, sometimes narrowed at the base, unicellular, thick- and smooth-walled, multi-guttulate.
Culture characters: colonies on PDA, white to pale grey in the middle region, white in the edges, velutinous to felty, flattened, dense, with undulate margin, reverse dark brown in the middle region, white in the edges.
Material examined: Thailand, Chiang Mai, on submerged decaying wood in freshwater water, 29 November 2010, Huang Zhang, D53 (MFLU 11-1105, holotype), ex-type living culture MFLUCC 11-0087.
Notes: Phylogenetic analyses based on LSU, ITS, rpb2, tub2 sequence data shows that Neopyrenochaeta annellidica and N. maesuayensis clustered with N. telephoni, but formed two separate branches (Fig. 253). Neopyrenochaeta annellidica and N. maesuayensis differ from N. telephoni (= Pyrenochaeta telephoni Roh. Sharma et al.) in conidiomatal setae. The former have one setae type distributed on all sides, while the latter has two types: one type stiff, long, mainly concentrated around the ostiole, the other short, soft, spread all over the surface (Crous et al. 2015c). Neopyrenochaeta annellidica has annellidic conidiogenous cells which are distinct from the phialidic type in N. maesuayensis (without acropleurogenous conidiogenous cells) and N. telephoni (having terminal and apical apertures). Therefore, N. annellidica and N. maesuayensis are introduced as new species.
Neopyrenochaeta annellidica (MFLU 11-1105, holotype) a, b Appearance of dark brown to black conidiomata on the host (the conidiomata with white conidial mass is in moist condition). c, f, g Sections of peridium. d, e Vertical sections of conidiomata. h, i Conidiogenous cells and developing conidia. j–l Conidia. m Germinating conidium. n–o Culture on PDA . Scale bars c, g = 20 µm, d–e = 100 µm, f = 50 µm, h–l = 10 µm, m = 20 µm, n–o = 25 mm.
Li WJ, McKenZie EHC, Liu JK, Bhat DJ, Dai DQ, Caporesi E, Tian Q, Maharachcikumbura SSN, Luo ZL, Shang QJ, Zhang JF, Tangthirasunun N, Karunarathna SC, Xu JC, Hyde KD (2020) Taxonomy and phylogeny of hyaline-spored coelomycetes. Fungal Diversity 100: pages279–801.