Alloneottiosporina thailandica W.J. Li & K.D. Hyde, Fungal Diversity 100, 327 (2020)
Etymology: Referring to Thailand, where the holotype was collected.
Parasitic on living leaves of grass, forming conspicuous, small, rounded, black conidiomata. Sexual morph: undetermined, Asexual morph: Conidiomata 100–140 µm diam., 80–140 µm high, pycnidial, separate, gregarious to confluent, globose to subglobose, semi-immersed, unilocular, glabrous, ostiolate, thick-walled. Conidiomatal wall 10–20 µm wide, composed dark brown to brown, thick-walled cells of textura angularis in the above half, becoming hyaline, thin-walled cells in the basal part. Ostiole single, inconspicuous, circular, centrally located. Conidiophores reduced to conidiogenous cells. Conidiogenous cells arising all around the wall of conidiomata, 3–6.5 × 3–7 µm, hyaline, enteroblastic, ampulliform or short cylindrical, determinate, smooth-walled. Conidia 10–17 × 3.5–4.5 µm (mean = 15 × 4 µm; n = 30), hyaline, ellipsoid to ovoid, with a broad and rounded apex, and a narrow and truncate base, 1-septate, slightly constricted at the septum, smooth and thick-walled, guttulate, bearing mucoid appendage at both ends; apical appendage tentaculiform, undulate or widely flared, arising from the partial mucoid sheath which initially enclosed the upper part of young conidium; basal appendage small, inconspicuous, slightly flared .
Culture characters: Colonies on PDA, reaching 5 mm diam. after 5 weeks at 20–25°C, circular to irregular, with sparse, white aerial mycelium, surface olivaceous-black, reverse similar in colour.
Material examined: Thailand, Chiang Rai, on living leaves of grass, 19 March 2015, Jing-Zu Sun, WJL0030 (MFLU 19-2896, holotype), ex-type living culture MFLUCC 15-0576.
Notes: Alloneottiosporina thailandica shares a similar conidia form with the generic type, A. carolinensis, but can be distinguished by the number of septa. Alloneottiosporina thailandica has a single septum, while A. carolinensis has 2–3 septa. Moreover, A. thailandica has smaller conidia than A. carolinensis [20–30(–32) × 6.5–8.5 µm (mean = 25 × 7.5 µm)] and A. infundibularis [15–34 × (8–)9–10 µm (mean = 24.5 × 9.5 µm)].
Alloneottiosporina thailandica (MFLU 19-2896, holotype) a Herbarium specimen. b, c Black conidiomata on the host. d, e Vertical sections of conidiomata. f Vertical section of peridium. k–l, g–j Conidiogenous cells and developing conidia. q–r Germinating conidia. m–p, s–v Conidia. w, x Culture on PDA. Scale bars: b–c = 200 µm, d–e = 100 µm, f = 20 µm, g–j, m–v, q–r = 5 µm, k–l = 10 µm, w–x = 2 cm.
Li WJ, McKenZie EHC, Liu JK, Bhat DJ, Dai DQ, Caporesi E, Tian Q, Maharachcikumbura SSN, Luo ZL, Shang QJ, Zhang JF, Tangthirasunun N, Karunarathna SC, Xu JC, Hyde KD (2020) Taxonomy and phylogeny of hyaline-spored coelomycetes. Fungal Diversity 100: pages279–801.